Some questions about PCR consumables
Why are PCR consumables generally made of PP?
Answer: PCR/qPCR consumables are generally made of polypropylene (PP), because it is a biologically inert material, the surface is not easy to adhere to biomolecules, and has good chemical resistance and temperature resistance (can be autoclaved at 121 degrees bacteria, can also withstand temperature changes during thermal cycling). These materials are usually in direct contact with reagents or samples, so high-quality materials and good processing techniques are required in the production and preparation process.
There are many Different volumes of PCR tubes/plates, how do I choose?
Answer: The volume of PCR tubes can mostly meet the requirements of PCR reaction. However, on the basis of meeting the experimental requirements, low-capacity tubes are preferred. Because the low volume reactor tubes/plates have less overhead space, thermal conductivity is improved and evaporation is reduced. And when adding samples, it is necessary to avoid adding too much or too little. Too much can lead to reduced thermal conductivity, spillage and cross-contamination, while too little can cause evaporative sample loss. You can choose a more suitable product according to the specific experimental requirements.
Why do some PCR tubes have flat caps and some have convex caps?
Answer: When the sample volume is small, single tube or connecting tube will be preferred. But when the reaction volume requirement is large, a single tube can reach 0.5mL.
Different colors of PCR tubes/plates, how should I choose?
Answer: For ordinary PCR reactions, it is possible to use transparent or colored PCR tubes, and colored and transparent PCR tubes are more helpful for sample classification management.
The thickness of the PCR plate/tube material varies. Is the thicker one more stable?
Answer: Generally divided into no skirt, half skirt, full skirt
Non-less Plate: Compatible with most PCR machines or qPCR machines, but not suitable for automation, applications. The stability during pipetting is not high and needs to be used with the plate holder.
Semi-skirt plate: suitable for labels or barcodes, and automated applications, and has good pipetting stability.
Full Skirt Plate: Ideal for automated laboratory applications, but also suitable for labels and application barcodes. It has good mechanical strength, can be applied to PCR instruments with protruding modules, and has high stability during pipetting.
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