The Magnetic Beads Nucleic Acid Extraction
1. What is the magnetic beads method?
The magnetic bead method, also called the pipetting method, is to extract nucleic acid by fixing magnetic beads and transferring liquid. Generally, the operation system controls the mechanical arm to realize the transfer. The extraction process is as follows:
2.What is the magnetic beads method process?
Lysis: Add the lysis solution to the sample, realize the mixing and full reaction of the reaction solution through mechanical movement and heating, cell lysis, and release of nucleic acid.
Adsorption: Add magnetic beads to the sample lysing solution and mix them thoroughly. The magnetic beads have a strong affinity for nucleic acids under high salt and low pH values to adsorb nucleic acids. Under the action of an external magnetic field, the magnetic beads are separated from the solution, Use the tip to remove the liquid and discard it to the waste tank, and discard the tip.
Washing: Remove the external magnetic field, replace with a new tip, add washing buffer solution, mix thoroughly to remove impurities, and remove the liquid under the action of an external magnetic field.
Elution: Remove the external magnetic field, replace with a new tip, add elution buffer, and mix thoroughly. The bound nucleic acid is separated from the magnetic beads to obtain purified nucleic acid.
3.Precautions
The installation environment of the instrument: normal atmospheric pressure, temperature 20-35℃, typical use temperature 25℃, relative humidity 10%-80%, unobstructed air flow is 35℃ or below.
Avoid placing the instrument near a heat source, such as an electric heater; at the same time, to prevent short circuits of electronic components, avoid splashing water or other liquids into it.
The air inlet and exhaust vents are located on the back of the instrument, and at the same time, avoid dust or fibers from gathering at the air inlet to keep the air duct unobstructed.
The nucleic acid extractor is at least 10cm away from other vertical surfaces.
Instrument grounding: In order to avoid electric shock accidents, the input power cord of the instrument must be grounded.
Keep away from live circuits: Operators are not allowed to disassemble the instrument without authorization. Replacement of components or internal adjustments must be completed by qualified professional maintenance personnel. Do not replace components when the power is turned on.
4.Features of Genuture magnetic beads method nucleic acid extractor
Able to realize automatic and high-throughput operation.
The operation is simple and fast.
Safety and environmental protection.
High purity and high yield.
No pollution and stable results.
Low cost, convenient for wide application.
Can process different types of samples at the same time.
Geneture Medical provide one stop solution for nucleic acid extraction, please do not hesitate to contact us for more.