What are deep well plates?
What is a deep well plate?
Deep well plates have many nomenclature variations, including microplate, microwell, and microtiter. A deep well plate is a flat plate that looks like a tray with multiple wells used as small test tubes. Well plates are typically mixed with 96,384 wells in a 2:3 rectangle, although other chamber configurations are available. Some other less common sizes are 6,24 wells. Although 96-well microplates are compatible with automated equipment, the 96-well format is the most commonly used well format because it can be used manually by laboratory technicians or researchers. A more common use of 96-well format microplates is in enzyme-linked immunosorbent assay technology.
What are deep well plates used for?
Deep-well plates are used for a wide range of applications including cloning experiments, sample cultures, and in tissue culture-based assay systems
What is the role of deep well plate in the research laboratory?
Well plates have become a standard tool in analytical research and clinical diagnostic testing laboratories. Enzyme-linked immunosorbent assays are common and form the basis of most modern human and animal medical diagnostic tests. The wells of a microplate can typically hold tens of nanoliters to several milliliters of liquid. Microplates are versatile and have become a standard tool in research laboratories.
What are the characteristics of deep well plates?
While microplates are made from a variety of materials, the most common material is polystyrene. Microplates are available in several colors, including white pigmented, black pigmented, and clear. Different color choices are an important consideration when performing specific assays. For example, white pigmented microplates are ideal for optical absorption or luminescence detection. Black stained microplates are ideal for fluorescent bioassays. Transparent microplates have excellent optical properties and can be used for colorimetric analysis, as well as in the field of cell culture and storage. The physical properties of polystyrene (PS) make it an excellent choice for microplate construction. Polystyrene can handle the wide variation in temperature required for various laboratory setups, such as thermal cycling applications in cold storage at -112 degrees Fahrenheit, where temperatures can fluctuate throughout pre-programmed steps. Polystyrene boards also have the ability to contain chemical compounds for long-term storage. Other types of materials used in the construction of microplates include polycarbonate, cycloolefin (polypropylene), glass, and quartz, although polystyrene is the most commonly used material for making plates.
What are deep well plates made of?
Injection molding is the most common manufacturing process for polystyrene, polypropylene and cyclic olefins. Vacuum forming is used to process soft plastics such as polycarbonate. Composite microplates, more complex plates such as filter plates, SPE plates, and advanced PCR plate designs use multiple components that are molded separately and assembled later in the manufacturing process.
The Importance of Deep Well Plates
Microplates may look unremarkable on the surface, but life without them can be very different. Well plates are used in virology, serology, microbiology and countless other life sciences and drug discovery laboratories. Microplates are a simple, relatively low-tech, cost-effective, unassuming and unassuming tool that can be found in laboratories around the world. Microplates save researchers time and money for expensive reagents that can be extremely expensive.
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