What is the nucleic acid extractor?
With the rapid development of biotechnology, with the application of PCR technology in various fields, including medical disease detection, agricultural GMO detection and many other applications, high-throughput sample nucleic acid extraction has become extremely urgent. There are various principles of automatic nucleic acid extraction instruments, and the price is also different. How to choose a suitable nucleic acid automatic extraction instrument will be analyzed today.What is a nucleic acid extractor?
Nucleic acid extraction instrument is an instrument that automatically completes the nucleic acid extraction of samples by applying the supporting nucleic acid extraction reagents.
From the perspective of extraction principle, there are spin column method and magnetic bead method; spin column method fully automatic nucleic acid extractor adopts the method of combining centrifuge and automatic pipetting device. The flux is generally 1-12 samples, and the time and manual The extraction is almost the same, it does not improve the efficiency, the price is not cheap, and the consumables are not universal, which is suitable for the laboratory with more money.
The principle of nucleic acid extraction by magnetic bead method:
Using the superpara magnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, molecular biology research and other fields.
Magnetic bead method automatic nucleic acid extraction instrument is divided into magnetic rod method and suction method. First, suction method, as the name implies, suction method is carried out by automatic pipetting device, and lysate and magnetic beads are added through automatic pipetting device. Adsorption; suction off the lysate, add rinsing buffer, magnetic beads adsorption, suction off the rinsing buffer, add elution buffer. There seems to be no problem in the steps, but the biggest problem with the suction method is that in order not to remove the magnetic beads when removing the waste liquid, the pipette cannot be too close to the magnetic beads to prevent the magnetic beads and the waste liquid from being sucked out, so that Every time the waste liquid is pumped, there is always a small part that cannot be completely removed, especially the device with the magnet at the bottom, because the magnetic beads are attracted to the bottom by the magnet, so the pipette cannot be too close to the bottom, so that the rinsing solution cannot be completely removed. Removal, the residual salt and ethanol in the rinsing solution will affect the subsequent elution efficiency and PCR success rate; the device with the magnet on the side will have less residual liquid, but the elution efficiency is also not good, relying on DNA self-dissolving to washing. In the debuffering solution, unless you wait for more than half an hour, some 96 automatic nucleic acid extraction workstations take 150 minutes for one round of extraction, and the same time for the 32 magnetic bar method can be carried out for 5 rounds.
Besides, the magnetic bar method nucleic acid extraction instrument usually has 8, 16, 32, and 96 channels of flux. Compared with the suction method, the advantage of the magnetic bar method is that the liquid does not remain in each step, because the magnetic bar only takes away the magnetic beads and transfers them. Go to the corresponding reaction well in the next step. In addition, the extraction instrument of the magnetic rod method should have more complete functions. For example, it can be heated. It is best to control the temperature of each heating tank independently. Different temperatures, another very important point is that the motor driving the magnetic rod should be able to drive the magnetic rod to quickly mix and stir the liquid, which is conducive to automation and helps to complete the cracking and rinsing.
Luoyang Geneture Medical independently developed and produced a magnetic bead nucleic acid extraction instrument. The instrument has a flux of 32, has 8 independent temperature-controlled heating tanks, and is equipped with an ultraviolet sterilization device. It adopts a linear motor, which can drive a magnetic rod to quickly stir the sample. The whole extraction time is 15-20 minutes, which is quite fast; it is worth mentioning that the consumables used in this instrument are general-purpose consumables, and ordinary 96 deep-well plates can be used. In this way, users can combine high-throughput sample grinding The instrument is used together with the instrument, and after grinding, it can be directly extracted by the nucleic acid extractor, which eliminates the tedious steps of sample transfer and saves a lot of time! Welcome new and old customers to consult!